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doi:.10.1002/hep.21752 [PubMed] [Cross Ref]28. Newberry EP, Xie Y, Kennedy S, Han X, Buhman KK, Luo J, Gross RW, Davidson NO. 2003. All rights reserved. The human liver fatty acid binding protein (FABP1) gene is activated by FOXA1 and PPARα and repressed by C/EBPα: implications in FABP1 down-regulation in nonalcoholic fatty liver disease. navigate here
Virus Res 115:31–42. Proc Natl Acad Sci U S A 107:9340–9345. Pharmacol Rev 56:291–330. [PubMed]43. doi:.10.1016/j.ajpath.2013.12.002 [PMC free article] [PubMed] [Cross Ref]26.
L-FABP T94A decreased fatty acid uptake and altered hepatic triglyceride and cholesterol accumulation in Chang liver cells stably transfected with L-FABP. Increasing evidence has indicated that chronic HBV infection, as well as HCV infection, is associated with hepatic steatosis through alterations in lipid metabolism (2, 3). However, the molecular mechanism underlying HBV-induced pathogenesis of hepatic steatosis still remains to be elucidated. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
- doi:.10.1073/pnas.84.4.1005 [PMC free article] [PubMed] [Cross Ref]29.
- doi:.10.1053/j.gastro.2007.03.039 [PubMed] [Cross Ref]5.
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- Zimmerman AW, Veerkamp JH. 2002.
- The enigmatic X gene of hepatitis B virus.
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- Luminescence measurement was carried out on a luminometer (Orion II microplate luminometer; Berthold Detection Systems, Germany).
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- doi:.10.1097/00041433-199405030-00006 [PubMed] [Cross Ref]12.
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P9767) and sodium oleate (catalog no. NCBISkip to main contentSkip to navigationResourcesHow ToAbout NCBI AccesskeysMy NCBISign in to NCBISign Out PMC US National Library of Medicine National Institutes of Health Search databasePMCAll DatabasesAssemblyBioProjectBioSampleBioSystemsBooksClinVarCloneConserved DomainsdbGaPdbVarESTGeneGenomeGEO DataSetsGEO ProfilesGSSGTRHomoloGeneMedGenMeSHNCBI Web We further observed that FABP1 was activated markedly in HBx-expressing hepatoma cell lines and HBx-Tg mice. doi:.10.1016/j.virusres.2005.07.003 [PubMed] [Cross Ref]38.
Powered sorof If you find Yahoo! Studies in HepG2 cells suggested that the overexpression of FABP1 markedly increased the rate of fatty acid uptake. doi:.10.1146/annurev.nutr.27.061406.093710 [PubMed] [Cross Ref]10. The metabolism of methylated aminoazo dyes.
pRep-HBV (harboring 1.2 unit lengths of the HBV genome) and control plasmid pRep-Sal were described previously (6). doi:.10.1128/JVI.74.1.83-90.2000 [PMC free article] [PubMed] [Cross Ref]39. The immune complexes were eluted and subjected to Western blot analysis with the respective antibodies.ChIP assay. Powered sorof is highlighted in the right pane of the Registry Editor window.
To further investigate the mechanism underlying HBx-enhanced FABP1 expression, we examined whether HBx could directly affect the expression of HNF3β, C/EBPα, and PPARα. Consistently, liver weights and liver TG contents were significantly higher in HBx-Tg mice than in WT mice and were reduced by LV-FABP1-shRNA treatment (Fig. 7D and andE).E). Proteomic screens showed FABP1 to be overexpressed in obese subjects with simple steatosis, along with paradoxically decreased expression in the progressive versus mild forms of nonalcoholic steatohepatitis (20).Given the key role This may not be the complete list of references from this article.
J Clin Invest 103:1489–1498. check over here Cardioprotective properties of fibrates: which fibrate, which patients, what mechanism? In Vitro. 1978 Mar;14(3):271–281. [PubMed]Stampfer M, Hallowes RC, Hackett AJ. Su F, Schneider RJ. 1996.
I sent Exterminate It a sample and the next day I received an e-mail instructing me to update and rescan. Liver tissues were fixed in 4% formalin and embedded in paraffin according to standard methods. Consistently, the cellular TG contents were significantly higher in Ad-HBx cells than in Ad-GFP cells (Fig. 6C), and siRNA-FABP1 treated cells markedly reduced TG accumulation. his comment is here Dramatically slowing down your computer.
smudge101 18:57 20 Nov 04 Did an online scan and there was a virus (TFTP1)Avast, a normally great program, was not working due to the latest version not being compatible with For immunoprecipitation, sonicated cell lysates were incubated with anti-HNF3β, anti-C/EBPα, anti-PPARα, or anti-Flag M2 (the same amount of normal IgG as control) antibodies for protein-DNA binding detection. Wu BK, Li CC, Chen HJ, Chang JL, Jeng KS, Chou CK, Hsu MT, Tsai TF. 2006.
The intracellular distribution of protein-bound azo dye in rat liver.
Mol Cell Biochem 219:127–138. The molecular mechanism by which HBV induces the pathogenesis of hepatic steatosis remains elusive. The C/EBPs and D site-binding protein in cell cycle control, carcinogenesis, circadian gene regulation, liver regeneration, apoptosis, and liver-specific gene regulation. J Virol 70:4978–4985. [PMC free article] [PubMed]40.
Paraffin-embedded tissues were sectioned (5 μm) and stained with hematoxylin and eosin (H&E) for morphological analysis.qPCR and Western blot analysis. Heterogeneity of tumor cells from a single mouse mammary tumor. After you delete a locked file, you need to delete all the references to the file in Windows registry. weblink Blocking of G1/S transition and cell death in the regenerating liver of hepatitis B virus X protein transgenic mice.
The putative binding sites of HNF3β, C/EBPα, and PPARα on the FABP1 promoter were shown in Fig. 4B (upper panel). Hepatic lipid accumulation was measured by using Oil-Red-O staining and the triglyceride level. J Natl Cancer Inst. 1973 Dec;51(6):1849–1860. [PubMed]Dexter DL, Kowalski HM, Blazar BA, Fligiel Z, Vogel R, Heppner GH. doi:.10.1016/j.bbrc.2005.12.089 [PubMed] [Cross Ref]25.
Yes, my password is: Forgot your password? doi:.10.1053/j.gastro.2007.04.061 [PubMed] [Cross Ref]2. Global proteome analysis of hepatitis B virus expressing human hepatoblastoma cell line HepG2. Cancer Sci 97:977–983.
These data suggest that FABP1 is elevated under the condition of HBV infection and may play an important role in HBV infection-induced hepatic diseases.FIG 1Effect of HBV on FABP1 mRNA and As expected, serum FABP1 levels were significantly higher in HBV-positive patients, with the HBV titer exceeding 107 copies/ml (Fig. 1E), and mice than in their control groups (Fig. 1F). Newberry EP, Kennedy SM, Xie Y, Luo J, Davidson NO. 2009. PMCID: PMC345298Epithelial mouse mammary cell line exhibiting normal morphogenesis in vivo and functional differentiation in vitro.K G Danielson, C J Oborn, E M Durban, J S Butel, and D Medina Copyright
Barnabas S, Andrisani OM. 2000. Thus, we hypothesized that HBx may be involved in HBV-induced hepatic steatosis.To address this hypothesis, we first investigated the effect of HBV on FABP1 expression in HBV-producing hepatoma cell lines and